Two yeast plasmids that confer nourseothricin-dihydrogen sulfate and hygromycin B resistance in Neurospora crassa and Cryphonectria parasitica.

Characterization of transferable plasmids from Shigella flexneri 2a that confer resistance to trimethoprim, streptomycin, and sulfonamides.

A set of plasmids conferring resistance to several antibiotics, including the combination of trimethoprim and sulfamethoxazole, has been isolated from Escherichia coli following conjugative cotransfer from a clinical isolate of Shigella flexneri 2a. One of the plasmids, pCN1, was shown by subcloning and DNA sequencing to carry a gene encoding a trimethoprim-insensitive dihydrofolate reductase i...

Quelling in Neurospora crassa.

Heterocaryosis in Neurospora Crassa.

T IS possible by means of X-ray or ultraviolet treatment to produce mutant I strains of Neurospora crassa that are deficient in their abilities to synthesize specific vitamins, amino acids, or other essential substances (BEADLE and TATUM 1941; TATUM and BEADLE 1g4zb). It occurred to us that it might be possible to determine whether the mutant genes concerned in such haploid strains are recessiv...

Biotransformation of Hydrocortisone by Neurospora crassa

The ability of Neurospora crassa FGSC 4335 in the biotransformation of hydrocortisone was investigated. The microorganism produced two major metabolites after incubation with the substrate for seven days. Each microbial product was purified chromatographically and identified on the basis of spectral data. The products were identified as 11?,17?,20?,21-tetrahydroxypregn-4-en-3-one (II) and 11?-h...

Characterization of Mobile Staphylococcus equorum Plasmids Isolated from Fermented Seafood That Confer Lincomycin Resistance

The complete nucleotide sequences of lincomycin-resistance gene (lnuA)-containing plasmids in Staphylococcus equorum strains isolated from the high-salt-fermented seafood jeotgal were determined. These plasmids, designated pSELNU1-3, are 2638-bp long, have two polymorphic sites, and encode typical elements found in plasmids that replicate via a rolling-circle mechanism including the replication...